Methods to identify target proteins of diverse ligands
Highly effective identification of drug targets at the proteome level by pH-dependent protein precipitation
Xiaolei Zhang, Keyun Wang, Sijin Wu, Chengfei Ruan, Kejia Li, Yan Wang, He Zhu, Xiaoyan Liu, Zhen Liu, Guohui Li, Lianghai Hu, Mingliang Ye
Chemical Science, 2022, 13, 42, 12403-12418
Solvent-Induced Protein Precipitation for Drug Target Discovery on the Proteomic Scale
Xiaolei Zhang, Qi Wang, Yanan Li, Chengfei Ruan, Shuyue Wang, Lianghai Hu, Mingliang Ye
Analytical Chemistry, 2020, 92, 1363−1371
Matrix Thermal Shift Assay for Fast Construction of Multidimensional Ligand−Target Space
Chengfei Ruan, Yan Wang, Xiaolei Zhang, Jiawen Lyu, Na Zhang, Yanni Ma, Chunzhen Shi, Guangbo Qu, Mingliang Ye
Analytical Chemistry, 2022, 94,17, 6482-6490
Proteome-Wide Deconvolution of Drug Targets and Binding Sites by Lysine Reactivity Profiling
Chengfei Ruan, Jiahua Zhou, Zhouxian Li, Kejia Li, Zheng Fang, Xiaolei Zhang, Mingliang Ye
Analytical Chemistry, 2022, 94, 3352−3359
Microparticle-Assisted Precipitation Screening Method for Robust Drug Target Identification
Jiawen Lyu, Chengfei Ruan, Xiaolei Zhang, Yan Wang, Kejia Li, Mingliang Ye
Analytical Chemistry, 2020, 92, 13912-13921
Methods for the analysis of protein glycosylation
Glyco-Decipher enables glycan databaseindependent peptide matching and in-depth characterization of site-specific N-glycosylation
Zheng Fang, Hongqiang Qin, Jiawei Mao, Zhongyu Wang, Na Zhang, Yan Wang, Luyao Liu,Yongzhan Nie, Mingming Dong, Mingliang Ye
Nature Communications, 2022, 13:1900, 1-15
Robust Glycoproteomics Platform Reveals a Tetra-Antennary Site-Specific Glycan Capping with Sialyl-Lewis Antigen for Early Detection of Gastric Cancer
Luyao Liu, Lei Liu, Yan Wang, Zheng Fang, Yangyang Bian, Wenyao Zhang, Zhongyu Wang, Xianchun Gao, Changrui Zhao, Miaomiao Tian, Xiaoyan Liu, Hongqiang Qin, Zhimou Guo, Xinmiao Liang, Mingming Dong, Yongzhan Nie, Mingliang Ye
Advanced Science, 2023, 2306955, 1-14
Endo-M Mediated Chemoenzymatic Approach Enables Reversible Glycopeptide Labeling for O-GlcNAcylation Analysis
Yao Chen, Feng Tang, Hongqiang Qin, Xuyang Yue, Yongzhan Nie, Wei Huang, Mingliang Ye
Angewandte Chemie-International Edition, 2022, e202117849, 1-9
O‑Glycopeptide Truncation Strategy for Heterogeneous O‑GalNAc Glycoproteomics Characterization
Luyao Liu, He Zhu, Lei Liu, Xin You, Jiawei Mao, Yan Wang, Xiaoyan Liu, Hongqiang Qin, Mingming Dong, Mingliang Ye
Analytical Chemistry, 2023, 95, 10017−10024
Development of an Integrated Platform for the Simultaneous Enrichment and Characterization of N- and O‑Linked Intact Glycopeptides
Zhongyu Wang， Zheng Fang, Luyao Liu, He Zhu, Yan Wang, Changrui Zhao, Zhimou Guo, Hongqiang Qin, Yongzhan Nie, Xinmiao Liang, Mingming Dong, Mingliang Ye
Analytical Chemistry, 2023, 95, 7448−7457
Methods for the analysis of protein methylation
Metabolic labeling based methylome profiling enables functional dissection of histidine methylation in C3H1 zinc fingers
Keyun Wang, Li Zhang, Sirui Zhang, Ye Liu, Jiawei Mao, Zhen Liu, Lin Xu, Kejia Li, Jianshu Wang, Yanni Ma, Jiayi Wang, Haitao Li, Zefeng Wang, Guohui Li, Hong Cheng, Mingliang Ye
Nature Communications, 2024, 15:7459, 1-14
A Chemoenzymatic Method Enables Global Enrichment and Characterization of Protein Arginine Methylation
Jiayi Wang, Qi Wang, Jiahua Zhou, Yan Wang, Zhen Liu, Keyun Wang, Mingliang Ye
Analytical Chemistry, 2024, 96, 14612−14620
Photoreactive Probe-Based Strategy Enables the Specific Identification of the Transient Substrates of Methyltransferase at the Proteome Scale
Zhen Liu, Keyun Wang, Mingliang Ye
Analytical Chemistry, 2023, 95, 12580−12585
Global profiling of arginine dimethylation in regulating protein phase separation by a steric effect–based chemical-enrichment method
Qi Wang, Zhouxian Li, Shenqing Zhang, Yichen Li, Yan Wang, Zheng Fang, Yanni Ma, Zhen Liu, Weibing Zhang, Dan Li,Cong Liu, Mingliang Ye
PNAS, 2022, 119, 43, e2205255119, 1-12
Methods for the analysis of cell surface proteins
Rapid Enzyme-Mediated Biotinylation for Cell Surface Proteome Profiling
Yanan Li, Yan Wang, Yating Yao, Jiawen Lyu, Qinglong Qiao, Jiawei Mao, Zhaochao Xu, Mingliang Ye
Analytical Chemistry, 2021, 93, 4542−4551
Temporal Tracking of Insulin Action on the Cell Surface of Proteins at a Resolution of Ten Seconds
Yanan Li, Yan Wang, Zheng Fang, Yan Liu, He Zhu, Yating Yao, Xin You, Hongqiang Qin, Mingliang Ye, Hailin Wang
Analytical Chemistry, 2023, 95, 9323−9329
Sensitive profiling of cell surface proteome by using an optimized biotinylation method
Yanan Li, Yan Wang, Jiawei Mao, Yating Yao, Keyun Wang, Qinglong Qiao, Zheng Fang, Mingliang Ye
Journal of Proteomics, 2019, 196, 33-41
Methods for the analysis of protein phosphorylation
A Tyrosine Phosphoproteome Analysis Approach Enabled by Selective Dephosphorylation with Protein Tyrosine Phosphatase
Xiaoyan Liu, Mingming Dong, Yating Yao, Yan Wang, Jiawei Mao, Lianghai Hu, Lishan Yao, Mingliang Ye
Analytical Chemistry, 2022, 94, 4155−4164
Ultra-deep tyrosine phosphoproteomics enabled by a phosphotyrosine superbinder
Yangyang Bian, Lei Li, Mingming Dong, Xuguang Liu, Tomonori Kaneko, Kai Cheng, Huadong Liu, Courtney Voss, Xuan Cao, Yan Wang, David Litchfield, Mingliang Ye, Shawn S-C Li, Hanfa Zou
Nature Chemical Biology, 2016, 12, 959-968
Robust phosphoproteome enrichment using monodisperse microsphere–based immobilized titanium (IV) ion affinity chromatography
Houjiang Zhou, Mingliang Ye, Jing Dong, Eleonora Corradini, Alba Cristobal, Albert J R Heck, Hanfa Zou, Shabaz Mohammed
Nature Protocols, 2013, 8,3,461-480
Specific Phosphopeptide Enrichment with Immobilized Titanium Ion Affinity Chromatography Adsorbent for Phosphoproteome Analysis
Houjiang Zhou, Mingliang Ye, Jing Dong, Guanghui Han, Xinning Jiang, Renan Wu, Hanfa Zou
Journal of Proteome Research, 2008, 7, 3957-3967
Methods for the large scale proteomics analysis
Reversed-Phase-Reversed-Phase Liquid Chromatography Approach with High Orthogonality for Multidimensional Separation of Phosphopeptides
Chunxia Song, Mingliang Ye, Guanghui Han, Xinning Jiang, Fangjun Wang, Zhiyuan Yu, Rui Chen, and Hanfa Zou
Analytical Chemistry,2010,82, 53-56
An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome
Yangyang Bian, Chunxia Song, Kai Cheng, Mingming Dong, Fangjun Wang, Junfeng Huang, Deguang Sun, Liming Wang, Mingliang Ye, Hanfa Zou
Journal of Proteomics, 2014, 96, 253-262